Comparison of in vitro (fish cell line) and in vivo (fish and crustacean) acute toxicity tests in aquatic toxicology

The use of in vitro (fish cell lines) is a cost-effective, very rapid, and informative tool for toxicological assessments. Using the neutral red (NR) assay, we compared acute toxicity (20hEC50) twenty-six chemical substances on rainbow trout gonad line (RTG-2) with their vivo to Barbados Millions Poecilia reticulata (48hLC50, OECD 203) crustacean Daphnia magna (48hEC50, 202). 20hEC50 values obtained by NR assay were higher nearly all cases when 48hLC50 P. 48hEC50 D. magna, indicating that sensitivity RTG-2 was lower magna. A high (r = 0.89) significant (P < 0.001) correlation recorded between 0.65; P 0.001), but also significant. authors recommend lines as screening protocol evaluate xenobiotics aquatic environments narrow spectrum concentrations fish test.